Some of these chromophores exhibit a high triplet state quantum yields that enable reactive oxygen species (ROS) production. Such agents are defined as photosensitizer. We have already shown that it became possible to use these molecules to disturb the membrane models in a fine and controlled way [1]. Our main goal today is to achieve greater levels of membrane complexity, to further explore the involved mechanisms, but also to strengthen their relevance to the biological point of view. Our aim is not only to change the composition of the model membranes on which we work, but mainly to better understand the relationship between membrane composition, its morphology and one specific biological activity. In this view, biomimetic membrane models such as proteo-liposomes (including i.e. ATPsynthase) are used [2] and structured illumination microscopy is now implemented in our lab for dynamic studies of mitochondrial membrane architecture [3]. Chemical characterization, kinetics of the photo-induced oxidation on phospholipid membrane, as well as intercellular communication can be studied by Raman and fluorescence spectroscopy of single nanoscale vesicles in the optical trap [4,5]. Moreover, taking into account the importance of oxygen in membrane oxidation processes, we have developed an original approach using both micro fluidic set-up and frequency domain fluorescence lifetime laser sheet microscopy in order to monitor oxygen gradient and diffusion in biological systems [6]